The procedures described here have potential applications for research and commercial utility to obtain plantlets in stage 1 tissue cultures of C. The success in recovery of plantlets from meristems and nodal explants is influenced by cannabis genotype, degree of endophytic contamination of the explants, and frequency of rooting. The callogenesis response of leaf explants of 11 genotypes on MS medium without activated charcoal was 35% to 100%, depending on the genotype organogenesis was not observed. sativa and has potential for obtaining pathogen-free plants. The development of plantlets from meristems is described for the first time in C. Following acclimatization, plantlet survival in hydroponic culture, peat plugs, and rockwool substrate was 57, 76, and 83%, respectively. Sodium metasilicate improved the visual appearance of the foliage and improved the rate of rooting. To obtain rooted plantlets, shoots from meristems and nodal explants of genotype Moby Dick were evaluated for rooting, following the addition of sodium metasilicate, silver nitrate, indole-3-butyric acid (IBA), kinetin, or 2,4-D.
The effect of Driver and Kuniyuki Walnut (DKW) or MS basal salts in media on shoot length and leaf numbers from nodal explants was compared and showed genotype dependency with regard to the growth response.
Seven commercially grown tetrahydrocannabinol (THC)-containing cannabis genotypes (strains) showed significant differences in response to shoot growth from meristems and nodal explants on Murashige and Skoog (MS) medium containing thidiazuron (1 μM) and naphthaleneacetic acid (0.5 μM) plus 1% activated charcoal. Various sterilization methods were tested to ensure shoot development from nodal explants by limiting the frequency of contaminating endophytes, which otherwise caused the death of explants. In this research, we evaluated variables that can influence the success of shoot growth and plantlet production in tissue cultures of drug-type Cannabis sativa L. Tissue culture approaches are widely used in crop plants for the purposes of micropropagation, regeneration of plants through organogenesis, obtaining pathogen-free plantlets from meristem culture, and developing genetically modified plants. Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.Holmes, Samantha Lung, Danielle Collyer and Zamir K. The default sky uses measured sky conditions as recorded by local weather stations.Janesse E. This information is used to perform snapshot analysis of daylight in your project. Updated LLF Calculator is back to help you determine LLF values based on IES guidelines.ĭaylight features add the ability to cut window openings, select location, sky condition and time of day. Searchable database of IES illuminances recommendations. Product Selection now includes a cloud-based option to access product data, eliminating the need to download large product files to your computer. The import/export capabilities have been updated to support the latest AutoCAD files using Autodesk's RealDWG conversion library. The lighting calculations have been updated to support multiple computer processors and decrease the calculation time for large design projects.
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